Web5 jun. 2024 · The HoxB8-FL cell line was recently reported to have both myeloid and lymphoid potential . We therefore also generated expression profiles for 107 HoxB8-FL single cells, which, when projected onto the diffusion plot, confirmed that the HoxB8-FL expression state resembles that of primary bone marrow cells from the LMPP trajectory. Web26 okt. 2024 · Hoxb8-FL cell line is a model system of lympho-myeloid progenitors, which self-renews in vitro and is amenable to genetic perturbations. To construct a functionally …
In Vitro Megakaryocyte Potential Hoxb8 Fl Cells Lonza Bioz
Web5 mrt. 2024 · Solene Accarias, Thibaut Sanchez, Arnaud Labrousse, Myriam Ben-Neji, Aurélien Boyance, Renaud Poincloux, Isabelle Maridonneau-Parini, Véronique Le Cabec, Ana-Maria Lennon-Duménil; Genetic engineering of Hoxb8-immortalized hematopoietic progenitors – a potent tool to study macrophage tissue migration. WebHoxb8 is a homeobox gene that inhibits myeloid differentiation. Integration of a murine stem cell virus encoding HoxB8 under the control of mutant estrogen receptor promotes … fenway bleacher 40
Phenotype of Cas9-Hoxb8 cell-derived DCs after LPS activation.
WebHoxb8-FL cells were transduced with MLL-ENL or empty virus to generate ME-Parental and Parental cells, respectively. When cultured in methylcellulose without Flt3L and estradiol (causing... Web1 jan. 2024 · Flow cytometry and single-cell transcriptomic characterisation of cells at different time points revealed that the mutation stalls the multipotent HOXB8-FL cell line along the differentiation trajectory resulting in a self-renewing progenitor-like state exhibiting haematopoietic markers indicative of a plasmacytoid dendritic cell-like phenotype. WebThese cells, referred to as Hoxb8-FL cells, retain lineage potential for lymphocyte and myeloid lineages, including the DC lineage. Upon removal of estrogen (inactivation of Hoxb8), Hoxb8-FL cells differentiate into highly homogenous DC populations in the presence of GM-CSF or FLT3L akin to their endogenous counterparts. fenway bleacher 38