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WebJournal: STAR Protocols Article Title: Optimized electroporation of CRISPR-Cas9/gRNA ribonucleoprotein complex for selection-free homologous recombination in human pluripotent stem cells doi: 10.1016/j.xpro.2024.100965 Figure Lengend Snippet: MaxCyte ATx mediated electroporation of RNP complex into iPSCs (A) Prepare a mixture of … WebHyClone™ Fetal Bovine Serum (FBS) are not only certified and verified for quality, but traceable back to their original source, whether from the US, Australia, or New Zealand. … WebFigure Legend Snippet: MaxCyte ATx mediated electroporation of RNP complex into iPSCs (A) Prepare a mixture of MaxCyte HyClone buffer with cells and RNP complex, and ssODN if applicable. Transfer 50 μL of the mixture into the well of OC-100 Processing Assembly chamber, after opening the lid. Then close the lid after the sample has been added to the … perth airport location