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Phis2

http://www.coolaber.com/Column.asp?Model=Content_Detail&Column_ID=29596&C_ID=284549825 WebbpHIS2 can be maintained in both yeast and bacteria. It contains an autonomous replication se-quence ( ARS4) and TRP1 nutritional marker for replication and selection in yeast (1, …

Functional dissection of a strong and specific microbe‐associated ...

Webb27 mars 2024 · The Y187 yeast strains containing recombined pHIS2 vectors were first cultivated in −T−H screening medium with different concentrations of 3-amino-1,2,4-triazole (3-AT), which was used to suppress background histidine leakiness of the pHIS2 vector. The concentration of 3-AT that prevented yeast growth in −T−H screening … http://wolfson.huji.ac.il/expression/bac-vectors.html how far is conover nc from me https://southwestribcentre.com

The walnut transcription factor - BMC Plant Biology

Webb12 apr. 2024 · （4）缺乏症：幼叶先皱缩变形、呈钩形、顶芽溃烂坏死，为不可再利用元素。 6 镁 （1）吸收形式：mg2+ （2）存在形式：mg2+、有机化合物 （3）生理功能：酶的活化剂、叶绿素的组分、和rna、dna、蛋白质的合成有关。 （4）缺乏症：老叶先开始缺绿，为可再利用元素。 WebbpHIS2 Description pHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterizeDNA-binding proteins. The vector was specifically designed for … Webb16 feb. 2024 · The product was cloned into the pHIS2 vector for the bait construction. The coding sequence of PhOBF1 with full length was introduced into the pGADT7-Rec vector harboring the GAL4 activation domain, which was regarded as the pray construct. The recombinant plasmids were co-transformed into Saccharomyces cerevisiae strain Y187. how far is connecticut to michigan

petunia transcription factor, PhOBF1, regulates flower senescence …

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Phis2

pADL-23c Phagemid - Antibody Design Labs manual.pdfThe …

WebbDescription. District Health Information Software 2 Tracker (DHIS2 Tracker) is an open-source, web-based application that supports data collection, and analysis of … http://coolaber.com/Column.asp?Model=Product_Detail&P_ID=1949722590

Phis2

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Webb1 apr. 2024 · To investigate the binding of TaMYB44-D to starch biosynthesis-related genes, the promoter sequences of 12 selected genes were cloned in front of the reporter gene HIS3 to construct the pHIS2.1-Pro plasmids. The plasmids pHIS2.1-Pro and pGADT7-Sec2-TaMYB44-D were co-transfected into the Y187 yeast strain to perform the Y1H … WebbClose You have no items in your shopping cart.. Home; Plasmid; p53- His2 Model: PVT11251

WebbUSD $185.00. Matchmaker Insert Check PCR Mix 1 is a 2X pre-mix that allows quick and easy PCR directly from yeast colonies. The Mix contains a PCR polymerase, primers, … Webb1 feb. 2024 · To determine the optimal concentration of 3-amino-1, 2, 4-triazole (3-AT) to suppress background histidine leakiness of the pHIS2, three recombinant pHIS2 vectors were transformed into the yeast strain Y187 for subsequent growing on − Trp/−His (−T/−H) medium with different concentrations of 3-AT.

Webb10 aug. 2015 · The yeast one-hybrid assay was carried out using the Matchmaker one-hybrid system following the manual (Clontech, Palo Alto, CA, USA). The promoter … WebbpHIS2 is a reporter vector that can be used in yeast one-hybrid assays to identify and characterize DNA-binding proteins. The vector was specifically designed for use with the …

Webb1 dec. 2024 · d Growth of yeast cells on SD/-Trp-Leu-His supplemented with 100 mM 3-AT. pHIS2.1-ProGA2ox3 plus pGADT7 and pHIS2.1-ProGA2ox7 plus pGADT7 served as the negative controls.

Webb说明：文章内容仅供预览，部分内容可能不全，需要完整文档或者需要复制内容，请下载word后使用。下载word有问题请添加微信号:fanwen365或QQ：370150219 处理（尽可能给您提供完整文档），感谢您的支持与谅解。 higgins intune hand feeding formulaWebbY187-pHis2YeastOne-HybridMediaKit（酵母单杂培养基套装YM1010），含有用于Y187-pHis2系统 酵母单杂交文库筛选的全套培养基，性价比更高。培养基以非常方便使用的 … how far is connecticut to north carolinaWebbStandard cloning vector (phagemid excised from lambda ZAPII). The f1 (+) orientation allows rescue of sense strand ssDNA. pBluescript II KS(+) has a reversed MCS. higgins insuranceWebbpHIS2.1 DNA target TRP1 Kanr HIS3 Figure 2. One-hybrid library construction and screening. Your target bait sequence is cloned upstream of the His3 reporter in pHIS2.1. … higgins intune ferret food reviewWebbVector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community … how far is connecticut to ohiohttp://www.honorgene.com/uploads/files/202411/6181dfe1813d2.pdf higgins insurance hopkinsville kentuckyhttp://www.qualityard.com/upload/2024-12-17/dbd8dacfb1f8d0626e1c10bc4840ba05.pdf how far is constantia from cape town